THE PLACE OF C677T POLYMORPHISM IN THE MTHFR GENE IN THE FORMATION OF METABOLIC SYNDROME
Keywords:Metabolic syndrome, insulin resistance, abdominal obesity, myocardial infarction, C677T genetic polymorphism in the MTHFR gene.
The material for the molecular genetic study was peripheral blood of 183 patients with metabolic syndrome and myocardial infarction (the main group) and 155 conditionally healthy donors (the control group). The main group was divided into 3 subgroups: 64 patients with MS+MI, 61 patients with MS without MI, and 58 patients with MI without MS. Testing of the C677T polymorphism in the MTHFR gene was performed on a Rotor-Gene Q device (Quagen, Germany), using a commercial test kit of Syntol LLC (Russia). DNA regions were amplified by real-time polymerase chain reaction (PCR) using TaqMan probes. Statistical processing of the results was performed using the standard OpenEpi V.9. 2 application software package.